Welcome to the Peptide Protocols Guide
Select a peptide from the index on the left to view detailed dosing instructions, administration guidelines, and clinical applications.
BPC-157 (Body Protection Compound)
Primary Uses
Gut healing, tendon/ligament repair, anti-inflammatory effects, wound healing, localized tissue repair
Reconstitution & Storage
- Each vial contains 10 mg lyophilized peptide
- Reconstitute with 3 mL bacteriostatic water (BW)
- Final concentration: 10 mg ÷ 3 mL = 3.33 mg/mL
- Gently swirl to dissolve (do not shake). Label vial with date mixed
- Refrigerate immediately after mixing (36–46°F / 2–8°C). Do not freeze
- Use within 30 days of reconstitution. Discard if cloudy, discolored, or past 30 days
Dosing Protocols
A) Standard Daily Protocol
| Typical Dose | mL (at 3.33 mg/mL) | Frequency | Duration | Focus |
|---|---|---|---|---|
| 250–500 mcg | 0.075–0.15 mL | Once daily | 4–6 weeks, then reassess | Gut, tendon/ligament, localized repair |
B) Alternative Weekly Simplified Protocol (Per Clinic Guidance)
| Weekly Volume | Approx. mg delivered* | Frequency | Duration | Notes |
|---|---|---|---|---|
| 0.35 mL | ≈ 1.16 mg | Once weekly | 4–6 weeks, then reassess | Clinic-approved simplified option |
*Calculation: 3.33 mg/mL × 0.35 mL = 1.1655 mg (rounded ≈ 1.16 mg)
Injection Instructions (Subcutaneous)
- Wash hands thoroughly. Clean vial stopper with alcohol
- Draw desired dose using insulin syringe (units ≈ mL × 100)
- Clean skin (abdomen or thigh). Pinch a fold of fatty tissue
- Insert at 45–90° angle, inject slowly, then withdraw and apply light pressure
- Rotate sites each injection: lower abdomen quadrants & outer thighs
- Record date/time, dose, and site in your injection log
Safety Information
Mild redness is common. Call clinic if you experience persistent swelling, rash, dizziness, shortness of breath, or any other concerning symptoms. Do not use if pregnant, breastfeeding, or with active cancer. Dispose of all needles in a sharps container.
Site Rotation Guide
Abdomen:
- Divide lower abdomen into 4 quadrants (L/R × upper/lower)
- Stay 2 inches away from navel
- Cycle clockwise: Q1 → Q2 → Q3 → Q4
Thighs:
- Outer mid-thigh band (L/R)
- Alternate left/right each injection
Supplies Needed
- Alcohol prep pads (for vial stopper & skin)
- Insulin syringes: 29–31G · ½ inch
- Bacteriostatic water (BW) for reconstitution
- Sharps container for needle disposal
TB-500 (Thymosin Beta-4)
Primary Uses
Systemic healing, circulation improvement, muscle repair, wound healing, anti-inflammatory, cardiovascular protection
Reconstitution & Storage
- Each vial contains 10 mg lyophilized peptide
- Reconstitute with 3 mL bacteriostatic water (BW)
- Final concentration: 10 mg ÷ 3 mL = 3.33 mg/mL
- Gently swirl to dissolve (do not shake). Label vial with date mixed
- Refrigerate immediately after mixing (36–46°F / 2–8°C). Do not freeze
- Use within 30 days of reconstitution. Discard if cloudy, discolored, or past 30 days
Dosing Protocols
A) Standard Split Protocol
| Weekly Dose | mL Total (at 3.33 mg/mL) | Frequency | Duration | Focus |
|---|---|---|---|---|
| 2–5 mg/week | 0.6–1.5 mL total weekly | Split into 2 injections (e.g., Mon/Thu) | 4–6 weeks, then maintenance PRN | Systemic healing, circulation, muscle repair |
B) Alternative Weekly Simplified Protocol (Per Clinic Guidance)
| Weekly Volume | Approx. mg delivered* | Frequency | Duration | Notes |
|---|---|---|---|---|
| 0.35 mL | ≈ 1.16 mg | Once weekly | 4–6 weeks, then reassess | Clinic-approved simplified option |
*Calculation: 3.33 mg/mL × 0.35 mL = 1.1655 mg (rounded ≈ 1.16 mg)
Injection Instructions (Subcutaneous)
- Wash hands thoroughly. Clean vial stopper with alcohol
- Draw desired dose using insulin syringe (units ≈ mL × 100)
- Clean skin (abdomen or thigh). Pinch a fold of fatty tissue
- Insert at 45–90° angle, inject slowly, then withdraw and apply light pressure
- Rotate sites each injection: lower abdomen quadrants & outer thighs
- Record date/time, dose, and site in your injection log
Safety Information
Mild redness is common. Call clinic if you experience persistent swelling, rash, dizziness, shortness of breath, or any other concerning symptoms. Do not use if pregnant, breastfeeding, or with active cancer. Dispose of all needles in a sharps container.
Site Rotation Guide
Abdomen:
- Divide lower abdomen into 4 quadrants (L/R × upper/lower)
- Stay 2 inches away from navel
- Cycle clockwise: Q1 → Q2 → Q3 → Q4
Thighs:
- Outer mid-thigh band (L/R)
- Alternate left/right each injection
Supplies Needed
- Alcohol prep pads (for vial stopper & skin)
- Insulin syringes: 29–31G · ½ inch
- Bacteriostatic water (BW) for reconstitution
- Sharps container for needle disposal
BPC-157 + TB-500 Combination Protocol
Synergistic Benefits
This combination provides both localized tissue repair (BPC-157) and systemic healing support (TB-500) for optimal recovery from injuries, surgeries, or chronic conditions. The dual approach offers comprehensive tissue repair, reduced recovery time, and is optimal for complex injuries.
Understanding the Mechanisms
- BPC-157: Works locally at injury sites, promotes angiogenesis, protects gut lining, accelerates tendon/ligament healing
- TB-500: Works systemically throughout the body, improves circulation, reduces inflammation, supports muscle repair
- Combined Effect: Addresses both immediate local tissue damage and overall systemic healing response
Dosing Options
Option A: Daily BPC + Twice Weekly TB-500 (Standard Protocol)
- BPC-157: 250-500 mcg (0.075-0.15 mL) daily in the morning
- TB-500: 2.5 mg (0.75 mL) twice weekly (Monday & Thursday)
- Duration: 6-8 weeks
- Total Weekly Doses: 1.75-3.5 mg BPC-157 + 5 mg TB-500
- Best For: Active injuries, post-surgery recovery, chronic conditions
Option B: Simplified Weekly Protocol (Clinic-Approved Alternative)
- Monday: BPC-157 0.35 mL (≈1.16 mg)
- Thursday: TB-500 0.35 mL (≈1.16 mg)
- Duration: 6-8 weeks
- Important: Space injections 3-4 days apart
- Best For: Maintenance, prevention, mild conditions, or those preferring fewer injections
Reconstitution Instructions
- Each vial (BPC-157 or TB-500) contains 10 mg lyophilized peptide
- Reconstitute each with 3 mL bacteriostatic water
- Final concentration for both: 3.33 mg/mL
- Gently swirl to dissolve - do not shake
- Label each vial clearly with peptide name and date mixed
- Store separately in refrigerator (36-46°F / 2-8°C)
- Use within 30 days of reconstitution
Injection Guidelines
- BPC-157: Can inject near injury site for enhanced local effects (optional)
- TB-500: Can inject anywhere subcutaneously (systemic effect)
- Technique: Use insulin syringes (29-31G, ½ inch)
- Sites: Rotate between abdomen quadrants and outer thighs
- Important: Never mix peptides in the same syringe
Enhanced Results Tips
- Stay well-hydrated throughout the protocol
- Engage in light movement/stretching to enhance circulation
- Ensure adequate protein intake (0.8-1g per lb body weight)
- Consider supplementing with Vitamin C and collagen support
- Get quality sleep (7-9 hours) for optimal healing
- Avoid NSAIDs if possible - they may interfere with healing
Important Safety Notes
Do not use if pregnant, breastfeeding, or have active cancer. These peptides work through different but complementary mechanisms. Always follow your provider's specific recommendations. Report any unusual reactions immediately. Mild injection site redness is normal.
Expected Timeline
- Week 1-2: Initial anti-inflammatory effects, reduced pain
- Week 3-4: Noticeable tissue healing, improved mobility
- Week 5-6: Significant repair progress, enhanced function
- Week 7-8: Consolidation of healing, lasting improvements
Clinical Guidance
These peptides are provided under Be Well Lifestyle Centers protocols. Final dosing is individualized after provider consultation. For refills or questions, contact Be Well Lifestyle Centers Front Desk.
Semaglutide
Primary Uses
Weight loss, type 2 diabetes management, appetite suppression, metabolic health
Dosing Protocols
| Week | Dose | Frequency | Notes |
|---|---|---|---|
| 1-4 | 0.25 mg | Weekly | Starting dose |
| 5-8 | 0.5 mg | Weekly | Titration |
| 9-12 | 1.0 mg | Weekly | Therapeutic dose |
| 13+ | 1.7-2.4 mg | Weekly | Max dose if needed |
Administration Instructions
- Timing: Same day each week, any time of day
- Sites: Abdomen, thigh, or upper arm
- Storage: Refrigerate, do not freeze
- Titration: Increase dose slowly to minimize GI side effects
Side Effects & Management
- Nausea: Take with food, stay hydrated
- Constipation: Increase fiber and water intake
- Fatigue: Usually improves after 2-3 weeks
- If severe side effects, reduce dose temporarily
Tirzepatide
Primary Uses
Weight loss, type 2 diabetes, dual GIP/GLP-1 agonist, superior weight loss vs semaglutide
Dosing Protocols
| Week | Dose | Frequency | Notes |
|---|---|---|---|
| 1-4 | 2.5 mg | Weekly | Starting dose |
| 5-8 | 5 mg | Weekly | First titration |
| 9-12 | 7.5 mg | Weekly | Second titration |
| 13-16 | 10 mg | Weekly | Third titration |
| 17+ | 12.5-15 mg | Weekly | Max dose if needed |
Clinical Notes
Tirzepatide has shown superior weight loss compared to semaglutide in clinical trials. The dual mechanism provides enhanced metabolic benefits.
AOD-9604
Primary Uses
Fat loss, metabolic enhancement, cartilage repair, no effect on IGF-1 or insulin
Dosing Protocol
- Standard Dose: 250-500 mcg daily
- Timing: Morning on empty stomach
- Duration: 12-16 weeks cycles
- Administration: Subcutaneous injection in fatty areas
CJC-1295
Primary Uses
Increased GH/IGF-1, muscle growth, fat loss, improved sleep, anti-aging
Dosing Options
| Type | Dose | Frequency | Notes |
|---|---|---|---|
| CJC-1295 w/ DAC | 2 mg | Weekly | Long-acting |
| CJC-1295 no DAC | 100-200 mcg | 1-3x daily | Short-acting, often with Ipamorelin |
Administration Guidelines
- Timing: Take on empty stomach (avoid food 30 min before and 90 min after injection)
- Best Time: Before bedtime for optimal GH release
- Storage: Refrigerate after reconstitution
Ipamorelin
Primary Uses
GH release, muscle growth, fat loss, improved sleep, joint health, minimal side effects
Dosing Protocol
- Standard Dose: 200-300 mcg per injection
- Frequency: 1-3x daily (morning, post-workout, bedtime)
- Best paired with: CJC-1295 for synergistic effects
- Duration: 8-12 week cycles
- Cycling: 8 weeks on, 8 weeks off to prevent receptor desensitization
Tesofensine
Primary Uses
Weight loss, appetite suppression, increased metabolism, mood enhancement
Dosing Protocol
- Starting Dose: 0.25 mg daily
- Therapeutic Dose: 0.5-1 mg daily
- Timing: Morning with or without food
- Duration: 12-16 weeks, then break
- Note: Triple reuptake inhibitor - monitor mood/BP
Semax
Primary Uses
Cognitive enhancement, neuroprotection, ADHD, stroke recovery, immune modulation
Dosing Protocols
| Route | Dose | Frequency | Notes |
|---|---|---|---|
| Nasal (0.1%) | 50-100 mcg | 1-3x daily | 1-2 drops per nostril |
| Nasal (1%) | 500-1000 mcg | 1-2x daily | Higher concentration |
| Intranasal | 750-1000 mcg | Once daily AM fasted | Optimal cognitive enhancement |
| SubQ | 250-500 mcg | Daily | Alternative route |
Selank
Primary Uses
Anxiety reduction, mood improvement, immune enhancement, cognitive support, PTSD, ADHD
Dosing Protocol
- Starting Nasal Dose: 75 mcg per nostril (150 mcg total)
- Standard Nasal Dose: 75-200 mcg per nostril
- Frequency: 1-3x daily as needed
- SubQ Alternative: 250-500 mcg daily
- Duration: Can be used long-term
- Note: Non-sedating anxiolytic, excellent for anxiety/PTSD/ADHD
Cerebrolysin
Primary Uses
Alzheimer's, stroke recovery, TBI, cognitive enhancement, neuroprotection
Dosing Protocols
| Indication | Dose | Route | Duration |
|---|---|---|---|
| Cognitive Enhancement | 5-10 mL | SC | 5 days/week × 4 weeks |
| Neurodegenerative | 10-30 mL | SC | 5 days/week × 4 weeks |
Dihexa
Primary Uses
Cognitive enhancement, neurogenesis, synaptic growth, potential Alzheimer's treatment
Dosing Protocol
- Oral Dose: 10-20 mg daily
- Transdermal: 20-40 mg daily
- Duration: 4-8 week cycles
- Note: Potent neurogenic compound, use with caution
Thymosin Alpha-1
Primary Uses
Immune enhancement, chronic fatigue, hepatitis, cancer adjuvant, anti-aging
Dosing Protocol
- Standard Dose: 1.6 mg
- Frequency: 2x weekly
- High Dose: 1.6 mg daily for acute conditions
- Duration: 6-12 months or as needed
LL-37
Primary Uses
Antimicrobial, wound healing, immune modulation, biofilm disruption
Dosing Protocols
Protocol 1: General Antimicrobial/Immune Support
- SubQ Dose: 100-200 mcg daily
- Duration: 2-4 weeks
- Use: General immune support, mild infections
Protocol 2: Active Infection/Biofilm Disruption
- SubQ Dose: 500 mcg twice daily
- Duration: Until infection resolved (typically 2-4 weeks)
- Use: Active infections, SIBO, biofilm-related conditions
- Note: Powerful antimicrobial peptide with broad-spectrum activity
Kisspeptin
Primary Uses
Testosterone boost, fertility, libido enhancement, hypothalamic function
Dosing Protocol
- SubQ Dose: 100-200 mcg
- Frequency: 2-3x weekly
- IV Bolus: 0.3-1.0 nmol/kg for testing
- Duration: 4-8 weeks
PT-141 (Bremelanotide)
Primary Uses
Erectile dysfunction, female sexual dysfunction, libido enhancement
Dosing Protocol
- Starting Dose: 0.5-1 mg
- Standard Dose: 1.75-2 mg
- Timing: 45-60 minutes before activity
- Max Frequency: 8 doses per month
- Side Effects: Nausea, flushing common initially
Epithalon
Primary Uses
Telomerase activation, anti-aging, circadian rhythm, immune function
Dosing Protocols
- Standard Protocol: 10 mg daily × 10 days
- Alternative: 5 mg daily × 20 days
- Frequency: 2-3 cycles per year
- Route: SubQ or IM injection
- Note: Based on protocols by Dr. Khavinson and Dr. William Seeds
- Advanced Protocol: Some practitioners use 10 mg × 20 days for enhanced effects
GHK-Cu
Primary Uses
Skin rejuvenation, wound healing, hair growth, anti-inflammatory, antioxidant
Dosing Protocols
Injectable Protocol
- SubQ Dose: 1-2 mg daily
- Duration: 4-8 week cycles
- Note: Blue color is normal due to copper
Topical Protocol
- Concentration: 2-4% GHK-Cu cream/serum
- Application: Apply to clean skin 1-2x daily
- Areas: Face, neck, hands, or areas of concern
- Duration: Can be used continuously
- Benefits: Improved skin texture, reduced wrinkles, wound healing
- Note: May cause temporary blue/green tinting of skin
MOTS-C
Primary Uses
Metabolic regulation, exercise mimetic, insulin sensitivity, mitochondrial function
Dosing Protocol
- Standard Dose: 10 mg
- Frequency: 3x weekly
- Alternative: 5 mg daily
- Duration: 4-8 week cycles
- Best Time: Morning or pre-workout
KPV
Primary Uses
IBD, gut health, anti-inflammatory, antimicrobial, wound healing
Dosing Protocol
- Oral Dose: 200-500 mcg, 1-3x daily
- SubQ Dose: 200-500 mcg daily
- Duration: 4-8 weeks
- Note: Excellent for gut inflammation
VIP (Vasoactive Intestinal Peptide)
Primary Uses
CIRS/mold illness, pulmonary hypertension, anti-inflammatory, immune modulation
Dosing Protocols
Nasal Protocol (Primary Route)
- Concentration: 50 mcg per spray
- Dosing: 1 spray in each nostril, 4 times daily
- Schedule: Upon waking, midday, late afternoon, bedtime
- Total Daily Dose: 400 mcg (200 mcg per nostril daily)
- Duration: 30 days minimum, often 3-6 months for CIRS
- Storage: Refrigerate nasal spray bottle
Subcutaneous Protocol (Alternative)
- Dose: 50-100 mcg, 1-2x daily
- Duration: 30 days minimum
- Note: Monitor VCS testing if used for CIRS
SS-31 (Elamipretide)
Primary Uses
Mitochondrial dysfunction, chronic fatigue, aging, exercise performance
Dosing Protocol
- Standard Dose: 4-10 mg daily
- Frequency: Once daily
- Duration: 4-8 weeks
- Note: Targets cardiolipin in mitochondria
Tesamorelin
Primary Uses
GH release, lipolysis, body composition improvement, visceral fat reduction, lipodystrophy treatment
10-Week Protocol (Off-Label Wellness Use)
| Week | Volume to Draw | Dose Per Injection | Frequency |
|---|---|---|---|
| Week 1 | 0.3 mL | 1.5 mg | 5 days/week (Mon-Fri) |
| Week 2 | 0.35 mL | ||
| Weeks 3-10 | 0.4 mL |
Protocol Details
- Total Cycle Length: 10 weeks
- Administration: Subcutaneous injection in abdomen
- Storage: Refrigerate after reconstitution
- Weekend Break: No injections on Saturday/Sunday
Alternative Medical Protocols
- FDA-Approved Dose (Lipodystrophy): 2 mg once daily
- Standard Clinical: 1 mg twice daily or 2 mg once daily
- Duration: 3-6 months for these protocols
Clinical Benefits
- Significant reduction in visceral adipose tissue
- Improved body composition
- Enhanced IGF-1 levels
- Better lipid profiles
- Improved cognitive function in some studies
Melanotan I
Primary Uses
Skin tanning without UV exposure, photoprotection, treatment of erythropoietic protoporphyria
Dosing Protocols
Consciousness Protocol (Low Dose)
- Dose: 250-500 mcg daily
- Purpose: Gradual tanning, minimal side effects
- Duration: 2-4 weeks then as needed for maintenance
- Side Effects: Minimal at this dose
Tanning Protocol (Standard)
- Loading Phase: 1 mg daily × 7-10 days
- Maintenance: 1 mg 2-3x weekly
- UV Exposure: Optional but enhances results
- Duration: Continue until desired tan achieved
- Common Side Effects: Nausea, facial flushing, decreased appetite
- Note: Less libido effects compared to Melanotan II
Administration Tips
- Take before bedtime to minimize nausea
- Start with lower doses to assess tolerance
- Results visible in 1-2 weeks
- Tan develops from inside out (not surface bronzing)
The Wolverine Protocol
Ultimate Healing Stack
Named after the comic book character's rapid healing ability, this comprehensive protocol combines multiple peptides for maximum tissue repair and recovery.
The Complete Stack
| Peptide | Dose | Frequency | Purpose |
|---|---|---|---|
| BPC-157 | 500 mcg | Daily | Local tissue repair |
| TB-500 | 2.5 mg | 2x weekly | Systemic healing |
| GHK-Cu | 2 mg | Daily | Collagen/skin repair |
| Thymosin Alpha-1 | 1.6 mg | 2x weekly | Immune support |
| CJC-1295/Ipamorelin | 200/200 mcg | Nightly | Growth hormone support |
Protocol Schedule
Daily:
- Morning: BPC-157 (500 mcg) + GHK-Cu (2 mg)
- Bedtime: CJC-1295/Ipamorelin (200/200 mcg)
Monday & Thursday:
- Add TB-500 (2.5 mg)
- Add Thymosin Alpha-1 (1.6 mg)
Duration & Cycling
- Full Protocol: 6-8 weeks
- Break: 4 weeks minimum
- Maintenance: Can continue BPC-157 alone if needed
Important Considerations
This is an advanced protocol requiring multiple daily injections. Work with your healthcare provider to determine if this comprehensive approach is appropriate for your condition. Blood work monitoring recommended.